Methods
Department of Neuroanatomy
Staining techniques
- In situ hybridization (ISH)
- Chromogenic in situ hybridization (CISH)
- Fluorescent in situ hybridization (FISH)
- Immunolabeling
- Brightfield staining (DAB)
- Fluorescent stains
Behavioral analysis of mice
- Open field locomotion (Noldus)
- Novel object/texture recognition
- Y-Maze maze tests (texture discrimination)
- Chemogenetics
Electrophysiological methods
- In vitro patch clamp recordings (single to quadruple)
- In vivo patch clamp recordings
- Optogenetic stimulation
- Pharmacological stimulation
Electron microscopic methods
- Transmission electron microscopy
- Scanning electron microscopy
- Correlative light and electron microscopy
Stereotaxy
- Viral vectors for tracing, optogenetics, chemogenetics
Genetic methods
- Transgenic mouse models
- PCR
- Western blot
- In situ probe manufacturing
Light microscopic methods
- bright field / fluorescence microscopy
- confocal laser scanning microscopy (Zeiss LSM 880 with AiryScan Fast)
- High-resolution differential interference contrast microscopy (Zeiss LSM 880)
- In vivo 2-photon microscopy (Zeiss MP7)
Image processing and analysis
- Quantitative analyzes (ImageJ, Imaris)
- Deconvolution (AutoQuant X, Zeiss Zen)
- 2D mapping of immune/FISH markers (Neurolucida)
- 3D reconstruction of nerve cells (Neurolucida)
Data processing and analysis
- Signal
- R, MatLab
- GraphPad PRISM, Sigma Plot
![[Translate to Englisch:] Bildgebene Verfahren im Institut für Neuroanatomie Göttingen](/fileadmin/_processed_/2/8/csm_Forschung_6_tb_365dc511a0.jpg)
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